Fig. 4

CUL4B exacerbated bone loss and MAT accumulation induced by OVX. a Representative micro-CT three-dimensional reconstruction images of trabecular bone of femurs from WT and MKO mice after sham or OVX surgery. Scale bar, 0.3 mm. b–f Quantitative measurements of the trabecular bone mineral density (BMD) (b), bone volume/total volume (BV/TV) (c), trabecular number (Tb.N) (d), trabecular thickness (Tb.Th) (e) and trabecular separation (Tb.Sp) (f) of femurs from WT and MKO mice after sham (n = 7;6) or OVX surgery (n = 7;8) by micro-CT. g Representative H&E staining of femurs from WT and MKO mice after sham or OVX surgery. Scale bar, 200 μm. (h) Representative IHC images of OCN, osteoblast number (Ob.N/BS) and osteoblast surface (Ob.S/BS) in WT and MKO mice after sham (n = 5;5) or OVX surgery (n = 5;6). Scale bar, 40 μm. i ELISAs of serum OCN in WT and MKO mice after sham (n = 7;6) or OVX surgery (n = 7;8). j Alizarin red and calcein double fluorochrome labeling of trabecular bone in femur metaphysis from WT and MKO mice after sham (n = 5;4) or OVX surgery (n = 5;7). Scale bar, 20 μm. Representative images are shown with quantifications of the mineral apposition rate (MAR) and bone formation rate (BFR). k Adipocyte number per tissue area and area of adipocytes per tissue area measured based on H&E images of femurs from WT and MKO mice after sham (n = 5;5) or OVX surgery (n = 5;6). l Representative IHC of FABP4 and IOD quantification of WT and MKO mice after sham (n = 5;5) or OVX surgery (n = 5;6). Scale bar, 40 μm. Error bars represent standard errors. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.000 1; ns, no significance