Fig. 2
From: Identification of an intronic enhancer regulating RANKL expression in osteocytic cells
Dying osteocytic cells highly express RANKL. a,b Uniform manifold approximation and projection (UMAP) plots of the scRNA–seq (GSE154719)29 on tdTomato+ cells derived from bone tissues of Dmp1-Cre;Sp7+/+;tdTomato+ mice (n = 21 671 cells) (a) and Dmp1-Cre;Sp7flox/flox;tdTomato+ mice (n = 5 098 cells) (b). The dot plots in the right panels show the expression of selected osteocyte marker genes in the identified subclusters. c UMAP plot of the integrated data (n = 26 769 cells) and feature plots showing the expression of osteocyte marker genes at the single-cell level. The arrowheads indicate Osteocytic Cell Cluster 9. d,e Functional enrichment analysis (d) and regulatory target gene set analysis (e) comparing normal osteocytes and dying osteocytic cells. f Violin plots showing the expression of selected genes in dying and normal osteocytic cells
