Fig. 4
Identification of the contents of BEVs. a Electrospray ionization mass spectrum of BEV proteins showing the ions observed in the m/z range of 100–1 200. b Observed masses in the mass spectrum corresponded in mass to the tryptic peptide of the HMGB1 protein. The “X” symbol indicates the m/z 640.335 01 region analyzed in (a). c Protein expression of HMGB1 in EVs, BEVs and BEV-depleted EVs. d Quantitative analysis of the protein expression of HMGB1/β-actin. e Schematic representation of pyroptosis induced by HMGB1. The data are presented as the means ± standard deviations (n = 3). Statistical analyses were performed using one-way ANOVA with post hoc Tukey’s test. *P < 0.05, ***P < 0.001
