Fig. 1
PGE2 concentration in bone is positively correlated with mechanical load. a Enzyme-linked immunosorbent assay (ELISA) analysis of PGE2 level in bone at 10 different points of 12-week-old C57BL/6 mice. b Representative μCT images of 8 different point bones from 12-week-old C57BL/6 mice. c Quantitative analysis of trabecular bone fraction (BV/TV), trabecular bone thickness (Tb. Th) and cortical bone thickness (Ct. Th). d ELISA analysis of PGE2 level in bone at 10 different points of 13-week-old C57BL/6 mice with HU for 7 days. e Representative μCT images and quantitative analysis of trabecular bone fraction (BV/TV), cortical bone area (Ct. Ar) of talus from normal 13-week-old C57BL/6 mice or with HU for 7 days. Scale bars, 50 μm. Representative images of immunostaining of osteocalcin (Ocn) positive cells f and analysis of Ocn+ cells in the subchondral bone of talus from normal 13-week-old C57BL/6 mice or with HU for 7 days g. Scale bars, 50 μm. h Representative images of immunostaining and quantitative analysis of the density of TRAP+ cells in the subchondral bone of talus, tibia, and calcaneus from normal 13-week-old C57BL/6 mice or with HU for 7 days. Scale bars, 50 μm. n ≥ 5 per group. *P < 0.05, **P < 0.01, and N.S. indicates not significant. Statistical significance was determined by Student’s t-test
