Fig. 5

Identifying the profibrotic macrophages and antifibrotic macrophages. a The pseudotemporal trajectory analysis of MP0, MP1, MP2, and MP3. Arrows indicated the direction of pseudotemporal trajectory. b The Gene Ontology enrichment analysis of MP1, MP2 and MP3. c The Qusage analysis of enriched pathways of each cluster of MPs. The color indicates the mean pathway intensity (Red, high. Blue, low). Right, pathways. Bottom, clusters. d The KEGG analysis of enriched pathways of each cluster of MPs. The color indicates the mean pathway intensity (Red, high. Blue, low). Bottom, clusters. Right, pathways. e Primary human fibroblasts treated with conditioned media from MP2 (SPP1⁺macrphages) (n = 3) or SPP1^- macrophages (n = 3), qPCR of stated genes, expression relative to COL1A1 mean expression of control primary human fibroblasts (n = 3), Mean ± SEM. f Primary human fibroblasts treated with TGFβ1 and conditioned media from MP3 (FOLR2⁺macrophages) (n = 3) or FOLR2^- macrophages (n = 3), qPCR of stated genes, expression relative to COL1A1 mean expression of control primary human fibroblasts (n = 3), Mean ± SEM. g The pathway analysis of MP3