Fig. 7 | Bone Research

Fig. 7

From: Lumbar instability remodels cartilage endplate to induce intervertebral disc degeneration by recruiting osteoclasts via Hippo-CCL3 signaling

Fig. 7

Abnormal stress allows osteoclast recruitment in CEP to initiate remodeling through the Hippo signaling. a Heatmap of the osteoclast related genes expression in CEPCs in the 12% CTS group versus the control group. b Gene set enrichment analysis of the osteoclastogenesis realted genes in the 12% CTS group as compared to the control group. c Representative images of TRAP staining on CEP in each group. d Quantitative analysis of (c), Osteoclasts surface/CEP surface (Oc.S/CS). e Top. IF staining of Osteocalcin in CEP from the Ctsk-Cre::TdTomato mice with sham or LSI surgery. Bottom, representative IF staining images of Nestin1+ area (green) with DAPI (blue). f Left, TRAP staining of the BMMs after induction by using the supernatant of CTS-treated CEPCs supplemented with low-does RANKL. Right, quantative analysis of osteoclast number in each group. g Gene expression analysis of Acp5, Oscar and Calcr in BMMs treated with the supernatant of CTS-treated CEPCs

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