Fig. 2
Pdzk1KO mice exhibit aggravated symptoms of mechanical load-induced OA. a Representative images of SA-β-Gal staining, immunofluorescence staining of γH2AX and quantification analysis of positive chondrocytes as a proportion of the total chondrocytes and average number of foci in passage 6 chondrocytes from Pdzk1KO mice and controls. Scale bars: 25 μm (SA-β-Gal) and 5 μm (γH2AX). n = 5 per group. b Representative images of safranin O/fast green staining, immunofluorescence of p16ink4a and p21 and quantification analysis of OARSI scale and positive chondrocytes as a proportion of the total chondrocytes in articular cartilage of Pdzk1KO and Control male mice aged 18 months. Scale bars: 50 μm. n = 5 per group. c Representative scanning electron microscopy micrographs show the damage of cartilage surface. d Representative images of safranin O/fast green staining, IHC staining of PDZK1, MMP13, immunofluorescence of Col2a1, p16ink4a, p21 and quantification analysis of OARSI scale and positive chondrocytes as a proportion of the total chondrocytes in articular cartilage of Pdzk1KO and Controls at 8 weeks after DMM surgery and Sham group. Scale bars: 50 μm. n = 10 per group. Data are shown as mean + SD. ns not significant; *P < 0.05. **P < 0.01. IHC immunohistochemical, DMM destabilization of the medial meniscus, OA osteoarthritis, OARSI Osteoarthritis Research Society International
