Fig. 1

SREBP2 protects mice from bone loss. a–d Femurs from 12-week-old female SREBP2^WT and littermate control SREBP2^∆M mice were subjected to microCT scan analysis. Representative 3D images of trabecular bone (a) or cortical bone (c) of distal femurs were shown. Scale bars: 1 mm. b Trabecular bone parameters of femurs from SREBP2^WT (n = 10) and SREBP2^∆M (n = 17) mice. Bone volume/ tissue volume (Tb. BV/TV), trabecular space (Tb. Sp), trabecular thickness (Tb.Th), and trabecular numbers (Tb.N). d Cortical bone parameters of SREBP2^WT (n = 8) and SREBP2^∆M (n = 11) mice. Bone volume per tissue volume (BV/TV) and porosity. e, f Histological assessment of femurs from 12-week-old female SREBP2 ^WT and SREBP2^∆M mice (n = 8 per group). e Representative images. Decalcified sections of distal femurs of SREBP2 ^WT and SREBP2^∆M mice were shown after TRAP staining. Methyl Green staining was followed to indicate bone. Scale bars: 200 µm. f The osteoclast surface per bone surface (Oc. S/BS), the number of osteoclasts per bone perimeter (N. Oc/B.Pm), eroded surface per bone surface (ES/BS) were quantified. g, h Bone dynamic labeling analysis from 12-week-old female SREBP2 ^WT and SREBP2^∆M mice. g Representative images of calcein labeling. Scale bars: 200 µm. h Mineral apposition rate (MAR) and bone formation rate per bone surface (BFR/BS) (n = 6 per group). i The measurement of C-telopeptide of collagen type 1 (CTX) and Procollagen type I N-terminal propeptide (P1NP) from 12-week-old female SREBP2 ^WT and SREBP2^∆M mice (n = 10 per group). All data are shown as median and interquartile range. n.s., not significant; **P < 0.01, ***P < 0.001, ****P < 0.000 1 by two-tailed unpaired t test