Fig. 3
TrkA inhibition in transgenic TrkAF592A mice reduces hip associated HO by radiology. a Schematic of the experiment: TrkAF592A mice, susceptible to controllable TrkA inhibition via the small molecule 1NMPP1, were used. TrkAF592A animals underwent HO induction, receiving either 1NMPP1 or vehicle control by i.p. injection 24 h and 2 h pre-operation and 1NMPP1 by drinking water throughout the study. Mice were analyzed 3 weeks post-operative. b Representative roentgenography (XR) and microcomputed tomography (µCT) images of control and 1NMPP1 treated TrkAF592A mice. Red arrows and red colorization indicate HO areas. c Grading of HO after 1NMPP1 treatment according to a modified Brooker classification. d Quantitative μCT analysis of the total HO, including bone surface (BS), bone volume (BV). e Quantitative μCT analysis of the acetabulum associated HO. f Quantitative μCT analysis of femur associated HO. Dots in scatterplots represent an individual animal. n = 8. Scale bars: 1 mm. Data presented as mean ± 1 SD. Unpaired two-tailed Student’s t test was used for a two-group comparison. *P < 0.05, **P < 0.01
