Fig. 4
TrkA inhibition in transgenic TrkAF592A mice reduces hip associated HO by histology. a Schematic of the experiment. Mice were treated with 1NMPP1 or vehicle control throughout the study period. HO and nerve analysis at 3 weeks post-operative. b H&E and Alcian Blue/Alizarin Red staining of HO. Dashed black lines indicate the high magnification area of the acetabulum and dashed yellow lines indicate HO site. Cartilage appears blue and bone appears red. c Quantification of bone and cartilaginous area within the HO site. n = 9 animals per group. d, e Representative images and quantification of immunostaining of TUBB3+ nerves within acetabulum associated HO. Tile scans appear above, while high magnification images are below. f, g Representative images and quantification of immunostains for the sensory nerve marker PGP9.5 within acetabulum associated HO. h, i Representative images and quantification of immunostains for the sympathetic nerve marker tyrosine hydroxylase (TH). j, k Representative images and quantification of immunostaining for the neurotrophin nerve growth factor (NGF). Scale bars: 200 μm. Data presented as mean ± 1 SD. No differences were observed any parameter examined between male and female animals. Dots in scatterplots represent an individual animal. Unpaired two-tailed Student’s t test was used for a two-group comparison. **P < 0.01, ***P < 0.001
