Fig. 8

Reduced HO site innervation is associated with polarization of HO infiltrating macrophages. Assessments of macrophage polarization were performed in the acetabulum associated HO areas, 3 weeks post-injury. a, b Representative images of CD86, CD206 and F4/80 immunofluorescent staining among control or 1NMPP1 treated TrkAF592A mice. Tile scans appear above, while high magnification images are below. c, d Quantification of CD86⁺/F4/80⁺ (M1-like macrophage) and CD206⁺/F4/80⁺ (M2-like macrophage) cell density among control or 1NMPP1 treated TrkAF592A mice. e, f Representative images of CD86, CD206 and F4/80 immunofluorescent staining among Bup-LNP or control treated mice. Tile scans appear above, while high magnification images are below. g, h Quantification of CD86⁺/F4/80⁺ (M1-like macrophage) and CD206⁺/F4/80⁺ (M2-like macrophage) cell density among Bup-LNP or control treated mice. n = 3 per group. Scale bars: 200 μm. Data presented as mean ± 1 SD. Dots in scatterplots represent an individual mouse measurement. Unpaired two-tailed Student’s t test was used for a two-group comparison. *P < 0.05, **P < 0.01