Fig. 2

Periostin was primarily expressed by M2 macrophages in the bone defect. a Flow cytometry analysis of F4/80⁺ CD86⁺ macrophages (M1 macrophages) and F4/80⁺ CD86⁺ macrophages (M2 macrophages) isolated from the defect site in WT mice. Quantification of FACS results for the proportion of M1 or M2 macrophages in b total cells or c F4/80⁺ macrophages (n = 6). d The number of F4/80⁺ iNOS⁺ macrophages (M1 macrophages) and F4/80⁺ CD86⁺ macrophages in WT mice on PSD 10. Quantification of immunofluorescence analysis results for the proportion of M1 or M2 macrophages in e total cells and f macrophages (n = 6). g Quantitative of real-time PCR (RT-PCR) analyses of the expression of periostin, and M1 or M2 macrophages marker isolated from WT mice in M0, M1, and M2 macrophages (n = 3). h The number of periostin⁺ M1 or periostin⁺ M2 macrophages in the defect site of WT mice on PSD 10. i Quantification of immunofluorescence analysis results for the number of periostin⁺ M1 or periostin⁺ M2 macrophages (n = 6). j Western blot analysis of the expression of periostin in M0, M1, and M2 macrophages from WT mice. cb cortical bone, df defect site, bm bone marrow (n = 3). *P < 0.05; **P < 0.01; ****P < 0.000 1. Student’s t test. Data were mean ± SD