Fig. 8
From: Periostin+ myeloid cells improved long bone regeneration in a mechanosensitive manner
Transplantation of mechanically conditioned macrophages in an artificial periosteum membrane promoted bone regeneration. a Western blot analysis and quantitation of the expression of periostin after treated with fluid shear stress in BMDMs (n = 5). b Quantitative RT-PCR analyses of the expression of periostin after fluid shear stress treatment (n = 4). c Western blot analysis and quantitation of the expression of periostin after treated with TGF-β in BMDMs (n = 3). d Experimental design schematic for the isolation of macrophages from C57BL/6 J donors and transplantation at the defect site during bone healing. e 3D Micro-CT images and of quantification of the new bone accrual isolated from C57BL/6 J mice on PSD 10 after MTD surgery in PCL/HA, PCL/HA with BMDMs, PCL/HA with FSS-treated BMDMs, PCL/HA with TGF-β-treated BMDMs from C57BL/6 J mice (n = 6). f Immunofluorescence staining and quantification of the periostin expression, OSX+ cells, EMCN+ vessels and angiogenesis-osteogenesis coupling on PSD 10 (n = 6). cb cortical bone, bm bone marrow, df bone defect. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.000 1. Ordinary one-way ANOVA. Data were mean ± SD
