Fig. 8 | Bone Research

Fig. 8

From: Isovitexin targets SIRT3 to prevent steroid-induced osteonecrosis of the femoral head by modulating mitophagy-mediated ferroptosis

Fig. 8

Isovitexin against SIONFH by promoting osteogenesis. a HE staining was performed to assess the success of the model induction (The yellow arrow indicates empty lacunae). Scale bar = 50 μm. b After Micro CT scanning, two-dimensional imaging of the microstructure of the femoral head in each group of rats was obtained. Blue-white color represents high bone density, while red-purple color represents low bone density. c The bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular spacing (Tb.Sp) of the femoral head in each group of rats were measured (n = 5). d and e IHC staining of RUNX2 expression in each group of rats (n = 5). For regular images, scale bar = 500 μm, and for magnified images, scale bar = 100 μm. f WB analysis of RUNX2 and OCN expression in each group of rats (n = 3). Data were shown as mean ± SD. One-way ANOVA with Bonferroni multiple comparisons test was used for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.000 1

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