Fig. 2
Conditional deletion of Lrp1 in skeletal progenitors impairs early bone and joint formation. Representative images of H&E staining of E16.5 shoulder (a), E13.5-P0 elbow (b), E16.5-P0 knee (c) and P0 hip (d) sections of WT and Lrp1flox/flox/Prrx1Cre homozygote conditional KO (cKO) mice. Scale bar, 200 µm. H, humerus; R, radius; U, ulna; HZ, hypertrophic zone; O, primary ossification centre; F, femur; FH, femur head; T, tibia; AS, acetabulum socket. e and f, Representative images of immunohistochemical staining of TIMP3 (e) and CCN2 (e) and fast green counterstaining in E16.5 knee sections of WT and cKO mice. Regions delineated by the red squares in the panels have been magnified in the right panels. Scale bar, 200 µm. g and h, Length of femur and humerus mineralised bone (g) and body (h) of E18.5 (g) and P0 (gand h) WT and cKO mice. Circles represent individual mice and bars show the mean ± SD. P values were evaluated by 2-tailed Student’s t test. ****P < 0.000 1
