Fig. 4

MRTF-A inhibits Kidins220 expression, limiting AMPK phosphorylation and glycolysis. a Heat map of top 30 significantly differentially expressed genes in NPCs after CCG treatment. b Volcano plot of significantly differentially expressed genes (Foldchange ≥ 1.5, P < 0.05). c The mRNA level of Kidins220 in NPCs were treated with CCG or OE-MRTF-A. d The PCR image of Kidins220 promoter region (MRTF-A antibody was used for immunoprecipitation, with normal IgG as the negative control and input as the loading control). e Relative luciferase activity in HEK 293T cells treated with CCG for 24 h. f–h The levels of Kidins220 in NPCs treated with different concentrations of CCG, CD or OE-MRTF-A. i AMPK was immunoprecipitated from NPCs after CCG treatment, and the presence of AMPK, p-AMPK, and Kidins220 in the immunoprecipitates was evaluated. j IF images of p-AMPK in NPCs transfected with OE-Kidins220 for 24 h. k The levels of Kidins220, p-AMPK, AMPK, PLD1, PFKM, and PFKFB3 in NPCs transfected with OE-GFP or OE-Kidins220 for 24 h. l The levels of Kidins220, p-AMPK, AMPK, PLD1, PFKM, and PFKFB3 in NPCs transfected with sh-Kidins220 and treated with CCG for 24 h. m Schematic diagram of multiple treatments. White scale bar = 200 μm. *P < 0.05, **P < 0.01, ***P < 0.001