Fig. 4
LATS1 regulates the cellular distribution of ZBTB20 in chondrocytes. a Representative images of immunofluorescence staining of ZBTB20 in primary chondrocytes exposed to IL-1β at concentrations ranging from 1 to 5 ng/mL. n = 3 biological independent experiments. b Statistical analysis of the cellular distribution of ZBTB20 in indicated groups of cells in (a). n = 6 views per group for one biological replicate. The left panel is quantification of N/C ratio of ZBTB20. The right panel is the statistical analysis for ZBTB20 cellular distribution. c Western blot of ZBTB20 in nucleus or cytoplasm extraction of chondrocytes treated with IL-1β ranging from 0 to 5 ng/mL. d Curves showing the cellular distribution of ZBTB20 in (c). e Western blot of COL II, LATS1 and pLATS1 in chondrocytes exposed to IL-1β. f Statistical analysis of fluorescence signal of LATS1 and pLATS1 in articular cartilage from mice underwent Sham or DMM surgery for 2/4/8 weeks. n = 3 mice per group. g Co-immunoprecipitation of Fg-ZBTB20 and Myc-LATS1. h Proximity labeling with TurboID and TurboID-ZBTB20 in chondrocytes. i Statistical analysis of bands of LATS1 in (h). j Representative images of immunofluorescence staining of ZBTB20 in primary chondrocytes upon IL-1β or/and TRULI stimulation. n = 3 biological independent experiments. k Statistical analysis of N/C ratio of ZBTB20 in (j). n = 6, 6, 5 views for one biological replicate. c–e, g–i n = 3 biological independent experiments
