Fig. 6
Both TRULI and DAPA may serve as anti-OA drugs. a Schematic diagram of administration of TRULI and DAPA in mice in injury induced OA model. b Dot plot graph showing the behavior abnormality indicated by ratios of the parameters between the right hind paw and left hind paw in each group of mice. c Representative images of Safranin O/Fast Green staining of articular cartilage from indicated groups of mice. d Statistical analysis of OARSI scores, thickness of hyaline cartilage (HC), calcified cartilage (CC) and ratio of thickness of HC to CC in indicated groups of mice described in (a). a–d n = 7, 7, 8, 8, 9, 9 mice. e Representative images of Safranin O/Fast Green staining of cultured cartilage explants from patient exposed to IL-1β, supplement with increased dosage of TRULI or DAPA. f Statistical analysis of released GAG into medium by DMMB assay in cultures described in (e). e, f n = 3 biological independent experiments. g Dot plot graph of the top 10 enriched KEGG pathways of DEGs from each comparison in human primary chondrocytes. g n = 4 replicates. h Schematic diagram representing molecular mechanisms modulating inflammation and ECM maintenance via LATS1-ZBTB20-PTEN axis
