Fig. 3

KIAA1199 regulates glucose homeostasis and lipid metabolism. a Plasma levels of glucose under fasting and fed in female KIAA1199 KO and WT mice, n = 12–15/group. The glucose tolerance test (GTT) (b), insulin tolerance test (ITT) (c), the calculated area under the curve (AUC) for GTT and ITT (d) were checked in female KO (n = 6) and WT (n = 7) mice. e Western blot analysis of phosphorylated Akt (P-Akt, p473) and total Akt (T-Akt) in adipose, muscle and liver tissues after 7 min intraperitoneal injection of insulin or vehicle in female mice, n = 3. f Steady-state expressions of genes associated with insulin signaling in adipose, muscle and liver from female mice, n = 7. g–l Plasma levels of triglycerides, free fatty acids, glycerol, total cholesterol, adiponectin and leptin measured by ELISA in female mice, n ≥ 7. Three independent experiments had run for test, the representative data are shown. All data are represented as mean ± SD, the comparison between WT and KO groups are performed by two-tailed unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001