Fig. 1
From: Advances in spatial transcriptomics and its application in the musculoskeletal system
A Concise Workflow for Integrating ST into Musculoskeletal System Research and Overview of the Main ST Technologies. The figure highlights key considerations for conducting ST research in the musculoskeletal system, such as sample types, transcriptome coverage, and species compatibility, in order to select between imaging-based or sequencing-based technologies. It also illustrates the main ST technologies, which researchers can choose based on different technical parameters and commercial availability. However, it is important to acknowledge that the choice of technology is a complex process, and this workflow may not be universally applicable to all biological questions. Researchers should carefully weigh their options when making a technological choice. Furthermore, it should be noted that some probe-based sequencing platforms, such as GeoMx DSP, Visium V2, and Visium HD, are limited to near-whole transcriptome detection in humans and mice only. cDNA complementary DNA. Created in BioRender. Wang, H. (2025) https://BioRender.com/e71m592
