Fig. 2

IGF2BP3 regulates cell proliferation, migration, invasion, inflammatory cytokine release and autophagy in RA-FLS. a The Western blot results showing IGF2BP3 expression in RA-FLS after treatment with TNF-α or siIGF2BP3. b, c GSEA revealed the enriched signaling pathways in RA patients with high IGF2BP3 expression based on hallmark gene sets. d The effect on TNF-α, IL-17 and MMP3 expression in RA-FLS treated with TNF-α or siIGF2BP3. e The content of IL-6 secreted by RA-FLS. Quantification of scratch wound healing assays (f) and transwell assays (g) of RA-FLS treated with TNF-α or siIGF2BP3. h RA-FLS apoptosis was measured via an Annexin V-FITC/PI staining assay. i Flow cytometric analysis was performed to evaluate the cell cycle distribution of RA-FLS. j Western blot analysis of IGF2BP3, p62 and LC3 levels in RA-FLS. k Representative images of the RA-FLS expressing mCherry-GFP-LC3 after treatment with TNF-α or siIGF2BP3. l Western blot analysis of IGF2BP3, p62 and LC3 levels in RA-FLS treated with TNF-α or overexpressing IGF2BP3. m The expression of TNF-α, IL-17 and MMP3 in RA-FLS treated with TNF-α or overexpressing IGF2BP3. *P < 0.05, **P < 0.01, ***P < 0.001