Fig. 6
From: IGF2BP3-mediated m6A modification of RASGRF1 promoting joint injury in rheumatoid arthritis
RASGRF1-mediated mTORC1 activation plays a crucial role for IGF2BP3 to promote RA-FLS proliferation and M1 macrophage polarization. a Western blot analysis showing the levels of IGF2BP3, RASGRF1, ULK1, p-ULK1, S6K, p-S6K, p62 and LC3 in RA-FLS treated with LPS, OV-IGF2BP3 or siRASGRF1. b The expression of TNF-α, IL-17 and MMP3 in RA-FLS. c Western blot analysis of IGF2BP3, RASGRF1, ULK1, p-ULK1, S6K, p-S6K, p62 and LC3 in RA-FLS. d The expression of TNF-α, IL-17 and MMP3 in RA-FLS. The proportion of CD86+ cells (e) and ROS release level (f) in RAW264.7 cells after the treatment of LPS, OV-IGF2BP3 or siRASGRF1. Compared with NC, #P < 0.05, ##P < 0.01, ###P < 0.001. Compared with OV- IGF2BP3, ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001
