Fig. 3
MAPL knockdown restricts Drp1 SUMOylation and mitochondrial fragmentation. a Western blots showing the levels of MAPL, Bax, Bcl-2, cleaved caspase-3 and SUMO1 in TNF-α-treated NPCs transfected with si-MAPL. b, c Western blots showing the levels of Drp1, MAPL and SUMO1 in the cytosolic fraction and mitochondrial compartment of NPCs after MAPL knockdown. d Co-IP of SUMOylated Drp1 in NPCs after treatment with si-MAPL. e Mitochondrial ROS production in NPCs transfected with si-MAPL. f, g JC-1 staining and flow cytometry were used to assess the ΔΨm in NPCs after MAPL knockdown. h, i DCFH-DA staining and flow cytometry were used to detect ROS accumulation in NPCs. j, k Flow cytometry with Annexin V-FITC/PI staining was used to evaluate the apoptosis rate of NPCs transfected with si-MAPL. (n = 3; *P < 0.05, **P < 0.01, and ***P < 0.001)
