Fig. 3
Changes in microtubule bundles in mitotic chondrocytes in response to substrates with different stiffnesses. a Representative CLSM images showing changes in microtubule bundles in chondrocytes in response to soft/stiff substrates. The cyan arrows indicate microtubule connections between the two cells. b Top-view CLSM images showing morphological changes in the microtubules of individual chondrocytes in response to soft/stiff substrates. c Quantitative analysis of the total number of microtubules in the cytoplasmic region showed that more microtubule bundles were formed on the stiff substrate than on the soft substrate. The results are based on 30 cells from five independent experiments. d Representative CLSM images showing the distribution of microtubules in mitotic chondrocytes in response to soft or stiff substrates (n = 5). Boxed areas (right) indicate changes in microtubule bundles at the center of the two nuclei. White arrows indicate different morphologies and distributions of microtubule bundles in the center of the two immature nuclei at anaphase. e Quantitative analysis of the number of microtubule bundles at the center of the two nuclei in mitotic chondrocytes. The results are based on 15 cells from five independent experiments
