Fig. 3 | Bone Research

Fig. 3

From: Tissue and extracellular matrix remodeling of the subchondral bone during osteoarthritis of knee joints as revealed by spatial mass spectrometry imaging

Fig. 3

Site-matched comparison of OA vs. cadaveric medial tissues—candidate selection and validation. a Medial sections from cadaveric controls were compared with site-matched OA tissues. b Hierarchical segmentation of the top 500 m/z features identified cartilage (blue) and three bone subtypes: healthy bone (yellow), transitional layer (green), and diseased bone beneath cartilage loss (magenta). c Comparing healthy bone (yellow, present in both groups) with diseased bone (magenta, OA only) showed increased MALDI-MS peak intensities in diseased regions. Pairwise ROC analysis identified 31 discriminatory features (AUROC ≥ 0.85). d Candidate features from this analysis were validated in the full cohort (n = 4 per group), revealing significant OA-associated increases, including peptides from Collagen α1(I) (m/z 900.40) and Collagen α1(III) (m/z 1 626.75). *P < 0.05; see Table S2

Back to article page