Fig. 7 | Bone Research

Fig. 7

From: Tissue and extracellular matrix remodeling of the subchondral bone during osteoarthritis of knee joints as revealed by spatial mass spectrometry imaging

Fig. 7

Integration of Spatial MALDI-MSI, Synovial Fluid Proteomics, and Mouse Models for OA Biomarker Discovery. a Spatial mass spectrometry imaging (MALDI-MSI) of OA knee joints identified candidate proteins for mechanistic and biomarker studies. Future work will expand to larger TKA cohorts, including sex-specific analyses, with validation in vitro, in vivo, and in OA mouse models. b Synovial fluid proteomics (DIA-MS) from knees of OA patients (red triangles, Outerbridge >2) and controls (green circles, Outerbridge ≤2) revealed distinct clustering by PLS-DA. Donor 106 showed inter-knee severity differences. Differential analysis identified 216 significantly altered proteins (Q < 0.05, Log2FC > 0.58), including COL1A1 and COL6A3, also upregulated in OA subchondral bone by MALDI-MSI. c Regression analysis highlighted several ECM and known OA proteins (CRTAC1, POSTN) that significantly correlated with OA stage and found several markers that were consistently regulated in synovial fluid (DIA-MS) and subchondral bone (MALDI-MSI) (COL1A1, COL1A2, COL6A3)

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