Fig. 1
The effects of NBIF, ABIF and FBS on in vitro cultured hBMSCs. a Scheme of experimental procedures. b Morphology of hBMSCs in vitro cultured for 7 days (D7) or 28 days (D28) under indicated culture conditions. Note the formation of spheroid-like structures in NBIF culture condition. Scale bars, 100 μm. c Growth curve of hBMSCs cultured under different conditions. 1 × 105 hBMSCs were seeded in a 6-cm² dish. After reaching 80% confluence, cells were re-plated for expansion at a density of 3 000 cells/cm². The expansion fold change between MSCs in NBIF medium and fetal bovine serum (FBS) medium was expressed as the mean of three experiments from day zero (D0) to day 28 (D28). Data were expressed as mean ± standard error of the mean (SEM) of the fold change across three replicates for each group. P-values were obtained from an unpaired t-test; **P ≤ 0.01; ***P ≤ 0.001. d Immunophenotype analysis of hBMSCs after 14-day culture by flow cytometry using specific surface markers. The results shown were representative results. n.s: not significant. e Statistical data of flow cytometry. The percentage of positive cells was obtained from three independent experiments, expressed as mean ± standard error of the mean (SEM) of the percentages across three replicates for each group. P-values from the unpaired t-test; ns, not significant; *P ≤ 0.05. f–h Morphology (f), growth curve (g) and flow cytometry result (h) of hBMSCs after long-term culture. P, passage. Scale bar, 100 μm
