Fig. 5 | Bone Research

Fig. 5

From: Neonatal bone marrow interstitial fluid supports expansion and osteogenic ability of human bone marrow mesenchymal stromal cells

Fig. 5

NBIF-primed hBMSCs promote bone regeneration. a Scheme of the experimental design for creating mouse calvarial defect and delivering hydrogel or hBMSCs-hydrogel mix. 8-week-old B-NDG mice were used, 5 mice for each repair treatment. Bone regeneration was evaluated by micro-computed tomography (μ-CT) 7 weeks post-treatment. b Representative μ-CT images of bone regeneration in the calvarial defect. Horizontal and vertical lines in the left column indicated the dissection lines for coronal (middle column) and sagittal (right column) views, respectively. Typical unrepaired areas were indicated by arrows. Scale bar, 1 mm. c, d Bone mass density (BMD) (c) and bone volume fraction (BV/TV) (d) of regenerated tissues in the defect area at 7 weeks post-surgery. Data were expressed as mean ± standard error of the mean (SEM) across indicated replicates for each group (n = 5 mice). P-values were obtained from an unpaired t-test; **P < 0.01, ***P < 0.001, ****P < 0.000 1

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