Fig. 1 | Bone Research

Fig. 1

From: Modeling the chondrocyte-derived osteoblasts formation process reveals its molecular signature and regulation network

Fig. 1The alternative text for this image may have been generated using AI.

Postnatal endochondral bone development is related to growth plate cartilage cells. a–g Comparative images of 5- and 10-week-old mice, (5w, 5 weeks; 10w, 10 weeks) (n = 40). A) X-ray images of dissected femur bones. b Histology sections H&E Staining, (CC Columnar cartilage, yellow arrow, HC Hypertrophic cartilage, blue arrow). c Quantitative studies of bone size and size of the different growth plate areas during postnatal development (n = 2–5). d Histology characterization of growth plate area of 10-week-old mouse bone samples (Each specific staining is mentioned in each image. Safranin-O refers to Safranin-O/Fast Green staining. Dotted lines delimit trabecular bone and bone marrow). e Histology showing trabecular and cortical bone interconnected in 5-week-old mouse bone samples (Each specific staining is mentioned in each image), f Histology of 10-week-old mouse bone samples showing remains of cartilage matrix proteoglycan staining (Red at Safranin-O staining) at cortical bone. g micro-CT reconstruction of bone head area and a transversal section at red line, showing the interconnected nature of trabecular and cortical bone tissues. h Lineage tracing of SOX9+ cells. Schematic of tamoxifen treatment for the induction of tomato expression and representative images of histology immunostaining (n = 18)

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