Fig. 7

B2UKX5 induces significant transcriptomic regulation in the nucleus pulposus region. a Flowchart illustrating the experimental design for transcriptomic analysis in the natural aging model. Volcano plot of DEGs (b) and directional DEG counts (c) in NP tissue between the vehicle-treated and 15-month-old groups. Volcano plot of DEGs (d) and directional DEG counts (e) in NP tissue between the B2UKX5-treated and vehicle-treated groups. f Venn diagram illustrating the overlap of DEGs in B2UKX5-treated and aging-related datasets. g Number of genes inversely regulated by B2UKX5 treatment relative to aging. h GO analysis of DEGs downregulated in aging and upregulated by B2UKX5 treatment. i GO analysis of DEGs upregulated in aging and downregulated by B2UKX5 treatment. j List of representative DEGs with inverse regulation by B2UKX5 in the aging process