Fig. 1
From: AKR1C enzymes sustain therapy resistance in paediatric T-ALL
AKR1C1-3 are overexpressed/activated in T-ALLs from therapy-resistant patients. a Box plots showing expression of selected transcripts (as indicated) in MRDneg (n = 29) and MRDpos (n = 19) T-ALL samples at diagnosis. b Graphs reporting the linear correlation existing between the expression values of the single AKR1C isoenzymes. Correlation between the mRNA expression of AKR1C1 vs. AKR1C2 (left panel), AKR1C1 vs. AKR1C3 (middle panel), and AKR1C2 vs. AKR1C3 (right panel) are shown. c Western blot analysis of AKR1C1-3 protein expression in MRD subgroups. Relative densitometric values of bands normalised to GAPDH expression are reported below each protein analysed. d, e Box plot summarising the slope of linear coumberone conversion during time of T-ALL cells from MRDneg (n = 11) and MRDpos (n = 9) patients d and relative correlation with the mRNA expression of each AKR1C isoenzymes (AKR1C1-3) (n = 14). mRNA expression of AKR1C1 (left panel), AKR1C2 (middle panel), and AKR1C3 (right panel) vs. the calculated slope of linear coumberone conversion reactions are shown. e In correlation graphs, 95% confidence interval is indicated by dotted lines. Moreover, Pearson r and relative p values are reported
