Fig. 2

Chemerin decreases migratory and invasive capabilities of HCC cells. Migratory and invasive capabilities of chemerin-overexpressing 7404 cells (a) and chemerin knockdown 7404/che H cells (b) are examined by Boyden Chamber Assay (upper) and Transwell Invasion Assay (lower), respectively. Quantitation is provided graphically (right), and each column represents the mean (±s.e.m.) number of cells from six randomly selected fields. *p = 0.0142 (migration) and **p = 0.0060 (invasion) for 7404/che L vs. 7404/v, ***p < 0.001 for 7404/che M, H Vs 7404/v, and 7404/che H i1, i2 vs. 7404/che H icon, Unpaired t test. c Effect of antibody (Ab) neutralisation on migration (1 μg/ml and 10 μg/ml Ab) and invasion (5 μg/ml and 50 μg/ml Ab) of 7404/che H cells. For migration, **p = 0.001 for 1 μg/ml Ab vs. IgG con, ***p < 0.001 for 10 μg/ml Ab vs. IgG con, Unpaired t test with Welch correction. For invasion, **p = 0.0048 for 5 μg/ml Ab vs. IgG con, ***p < 0.001 for 50 μg/ml Ab vs. IgG con, Unpaired t test. Scale bar = 100 µm