Fig. 2
From: Angiopoietin-like protein 3 blocks nuclear import of FAK and contributes to sorafenib response
ANGPTL3 is required to maintain sorafenib sensitivity in RCC cells. a CCK8 assay of ACHN and 769-p cells transfected with sh-ANGPTL3-1, sh-ANGPTL3-2 or sh-NC after sorafenib treatment at the indicated concentrations for 48 h (n = 3). The IC50 values are shown in the lower histogram. b Western blot analysis of the indicated proteins in ACHN and 769-p cells transfected with sh-ANGPTL3-1, sh-ANGPTL3-2 or control sh-NC after sorafenib treatment at the indicated concentrations for 48 h (n = 3). GAPDH was used as a loading control. c Flow cytometry analysis of Annexin V-stained 769-p and ACHN cells transfected with sh-ANGPTL3-1, sh-ANGPTL3-2 or sh-NC after sorafenib treatment (5 μM) for 48 h (n = 3). Representative images (left) and average ratio of cell death (right) are shown. Results are presented as the means ± SD. *p < 0.05. See also Supplementary Figure 2
