Fig. 4
DAC treatment inhibits viability and motility of methylator colon cancer as well as suppresses colon cancer cell-derived xenograft growth. Viability curves of the hypermethylated colon cancer cells RKO (a) and HCT116 (b) treated with DAC or DMSO determined by cell titer. Cells were treated with DAC or DMSO and cell viability was monitored at the indicated time points. Transwell assays of RKO (c) and HCT116 (d) cells treated with DAC or DMSO. 50,000 cells were subjected to transwell assays for 24 h in corresponding media supplemented with 10% FBS. e Representative image of mice bearing HCT116 tumours treated with DAC (left) or DMSO (right). Photos demonstrated the tumour growth 30 days after injection. f Representative images of explants from DAC (left) or DMSO (right) treated mice. g Scatter plotting of the weight of explants with or without DAC treatment
