Fig. 2

Atorvastatin suppresses mesenchymal breast cancer cells. MCF-7 RFP, MDA-MB-231 RFP, and MDA-MB-231 RFP/Ecad were grown in the presence of atorvastatin for 72 h and total cell number was determined by crystal violet staining and normalized to the control (a). IC₅₀ values for each cell line are reported (a). MCF-7 RFP, MDA-MB-231 RFP, and MDA-MB-231 RFP/Ecad were seeded either at 7.5 × 10⁴ or 20 × 10⁴ cells per well and probed for their total cell levels of E-cadherin and vimentin (b). The expression of E-cadherin (c–e) and vimentin (f–h) in MCF-7 RFP (c, f), MDA-MB-231 RFP (d, g), and MDA-MB-231 RFP/Ecad (e, h) was probed by immunofluorescent imaging. The data are representative of three independent experiments. Scalebar = 50 µm