Fig. 6 | British Journal of Cancer

Fig. 6

From: Bcl-2-dependent synthetic lethal interaction of the IDF-11774 with the V0 subunit C of vacuolar ATPase (ATP6V0C) in colorectal cancer

Fig. 6The alternative text for this image may have been generated using AI.

Regulation of IDF-1174 sensitivity to conditionally reprogramed cells (CRCs) derived from patients with colon cancer. a Western blot analysis of Bcl-2 expression in the CRCs. The signal intensity of each protein was normalized to that of GAPDH. b, c Autolysosome formation in CRCs treated with 10 µM IDF-11774. Scale bar, 200 µm. Bars indicate the ratio of the average of LysoTracker-positive cells in different regions (n = 9) of the well. d, e Growth inhibition of CRCs expressing different levels of Bcl-2 in the presence of 1 nM bafilomycin A1 and 5 µM IDF-11774 or 5 µM chloroquine, as measured using IncuCyte ZOOM system. Data are presented as the single representative result of three experiments and as the mean ± S.D. In each experiment, each treatment was repeated in triplet wells, and each well was analyzed in 4 frames. f Combination index of IDF-11774 with bafilomycin A1 (BM) or chloroquine (CQ) in CRCs

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