Fig. 1

Immune checkpoint expression on PDACs. a Western blot assay revealed positive immunostaining for PD-1 in MIAPaCa-2 and PANC-1 cells. Jurkat cells and PD-1 overexpression cell lysate were used as positive controls. Negative control was empty vector cell lysate. ß-actin was used for loading control. b Immunofluorescent staining was performed for PD-1 and PD-L1 expression in MIAPaCa-2 and PANC-1 cells. Positive PD-1 and PD-L1 immunostaining was observed in both cell lines. Merged images with DAPI nuclear stains were also constructed for both PD-1 and PD-L1. c Immunofluorescence was performed to assess expression of PD-1 and PD-L1 in 3 PDAC PDOs. PD-1 and PD-L1 immunostaining was observed for both patients 1 and 3. However, PD-1 immunostaining was absent in PDOs from patient 2. The second row was a merge with DAPI nuclear staining