Fig. 2

IFNγ treatment upregulates PD-L2 mRNA and protein expression in MET-amplified tumours. a Real-time qPCR analysis of PD-L2 mRNA on MET-amplified human cancer cells upon 6 h treatment with IFNγ. Fold change values with respect to untreated controls (NT) reported in the graphs are mean ± SD calculated from three independent experiments (***, P ≤ 0.001). b Flow-cytometry analysis of PD-L2 expression on cell membrane of MET-amplified tumours upon 48 h treatment with IFNγ. Mean fluorescence intensity (MFI) values in the graphs are Mean ± SD calculated from three independent experiments (**, P ≤ 0.005). c Representative dot plots from one independent experiment showing the % of viable PD-L2-positive cells in the absence (NT) or presence of IFNγ