Fig. 2

GAS5 expression analysis in BlCa. a Box plot and bar graph presenting GAS5 levels and GAS5 levels ratio, respectively, in bladder tumours and matched adjacent normal tissue specimens of bladder cancer patients. p-Values calculated by Wilcoxon Singed Rank test. b ROC curve analysis of GAS5 levels for the discrimination of bladder tumours patients from matched adjacent normal specimens. p-Value calculated by the Hanley and McNeil method. AUC area under the curve, 95% CI 95% confidence interval. c, d Box plots presenting the correlation of GAS5 levels with tumour stage (c) and tumour grade (d) in the screening cohort. p-Values calculated by Kruskal–Wallis test (c) and Mann–Whitney U-test (d). e, f Box plots presenting the correlation of GAS5 levels with tumour grade (e) and tumour stage (f) in the Hedegaard et al. validation cohort. p-Values calculated by Mann–Whitney U-test (e) and Kruskal–Wallis test (f). g, h Box plots presenting the correlation of GAS5 levels with tumour grade (g) and tumour growth (histologic subtype) (f) in the TCGA provisional validation cohort. p-Values calculated by Mann–Whitney U-test