Fig. 3
From: HPV, tumour metabolism and novel target identification in head and neck squamous cell carcinoma
Bioenergetic profile of HPV+ve (UD-SCC-2 [SCC2] and UPCI:SCC90 [SCC90]) and HPV−ve (SCC-25 [SCC25], UM-SCC-89 [SCC89]) cell lines. a The oxygen consumption rate (OCR; left panel) and extracellular acidification rate (ECAR; right panel) were measured using an extracellular flux analyzer (Seahorse Bioscience) to estimate glycolysis and mitochondrial respiration respectively. Port injections at indicated times included glucose (5mmol/l), the ATP synthase inhibitor oligomycin A and finally FCCP to uncouple mitochondria or 2-DG to cease glycolysis. HPV−ve cell lines demonstrated significantly globally elevated OXPHOS and glycolytic rates (p < 0.0001). b Histograms showing significantly increased glycolytic capacity in HPV-ve vs HPV+ve cell lines. A significantly reduced OCR/ECAR ratio in HPV−ve compared to HPV+ve cell lines indicates the latter are more reliant on oxidative phosphorylation for ATP requirements than glycolysis (ANOVA, Tukey's multiple comparisons, p < 0.01 for all comparisons of HPV-ve vs HPV+ve cell lines). Error bars represent SD and experiments (n = 2) were performed with a minimum of triplicate well repeats. ns not significant, *p ≤ 0.05; **p < 0.01; **p < 0.001; and ****p < 0.0001
