Fig. 4

SCC tumours catabolise glutamine via transaminase reactions and reductive carboxylation. a A diagram shows how [U-¹³C,U-¹⁵N]-glutamine would produce malate m+4 and then aspartate m+4 or m+5. Percent enrichment of malate, glutamate and aspartate isotopologues in slices of tumours and NC lung incubated with [U-¹³C,U-¹⁵N]-glutamine for 24 h. b Percent enrichment of citrate Isotopologues from slices in a. A diagram shows [U-¹³C,U-¹⁵N]-glutamine would produce citrate m+4 and m+5. Data are shown as box and whisker plots where whiskers represent the min and max. *P < 0.05 and **P < 0.01. AdC and SCC tumours were compared to their paired NC counterparts using the paired or Welch t-test, respectively. To test for significance between AdC and SCC, the Welch t-test was performed on the log-ratio of the tumour to their paired NC. See also Table S5