Fig. 4
Anti-HGF antibody abolished the ability of M2-CM to promote the proliferation, colony formation and migration of hepatoma cells. a After incubation of SMMC-7721 and Hep3B cells with 3 μM sorafenib in the presence or absence of anti-HGF antibody (aHGF) (200 ng/ml) for 48 h, cell proliferation was examined by CCK-8 assays. b Anti-HGF antibody abolished the effect of M2-CM on sorafenib inhibition of the colony formation of SMMC-7721 and Hep3B cells. c Anti-HGF antibody abolished the effect of M2-CM on the sorafenib inhibition of the migration of SMMC-7721 and Hep3B cells. N = 3, mean ± SEM, **P < 0.01, ***P < 0.001, NS, no significance
