Fig. 1
Construction of the chimeric antigen receptor CAR-147 targeting HER2. a The design of a chimeric antigen receptor (CAR-147) for macrophages is shown. b Human HER2 surface expression on 4T1 cells was measured. c CAR-147 surface expression on Raw264.7 cells was measured. d Control Raw264.7 (CON) cells and CAR-147 Raw264.7 (147) cells were cocultured with HER2-4T1 (HER2) cells at a ratio of 1:1 for 24 h, and the expression of MMP genes was assessed by RT-qPCR (n = 6). e Control Raw264.7 (CON) cells and CAR-147 Raw264.7 (147) cells were cocultured with HER2-4T1 (HER2) cells at a ratio of 1:2 for 24 h, and the expression of MMP genes was assessed by RT-qPCR (n = 6). f Control Raw264.7 (CON) cells and CAR-147 Raw264.7 (147) cells were cocultured with HER2-4T1 (HER2) cells at a ratio of 1:2 for 24 or 48 h, and the expression of MMP genes was assessed by RT-qPCR (n = 6). g Control Raw264.7 (CON) cells or CAR-147 Raw264.7 (147) cells were cocultured with wild-type 4T1 (CON) cells or HER2-4T1 (HER2) cells at a ratio of 1:1 for 24 h, and the expression of MMP genes was assessed by RT-qPCR (n = 6). All values are expressed as the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001
