Fig. 3 | British Journal of Cancer

Fig. 3

From: YY1 targets tubulin polymerisation-promoting protein to inhibit migration, invasion and angiogenesis in pancreatic cancer via p38/MAPK and PI3K/AKT pathways

Fig. 3

YY1 combined with the promoter region of TPPP regulates the expression of TPPP. a, b The TPPP expression in PANC-1-YY1 shRNA and BxPC-3-YY1 shRNA cells was measured by quantitative RT-PCR and western blot. c The cchematic diagram of the luciferase reporter construct containing the human TPPP promoter (pTPPP) and the mutant construct (pTPPP-YY1-M) containing the TPPP promoter in which the presumed YY1 binding site was mutated. d Luciferase assays demonstrating the luciferase activity of pTPPP (NC, WT or MUT) in PANC-1 (left) and BxPC-3 (right) cells transfected with YY1-overexpressing lentiviruses or control lentiviruses. Each error bar indicates the variation between the means of three independent experiments. e ChIP assays were performed in PANC-1-YY1 (left) and BxPC-3-YY1 (right) cells. Lane 1, DNA marker; lane 2, input DNA; lane 3, DNA from BxPC-3-YY1 cells immunoprecipitated with normal rabbit IgG; lane 4, DNA from BxPC-3-YY1 cells immunoprecipitated with an anti-YY1 antibody. (*represents p < 0.05, **represents p < 0.01, compared with the control group)

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