Fig. 2

TNC-induced enhanced ES cell proliferation, migration and angiogenesis were confirmed in vitro, and TNC-induced ES cell metastasis was confirmed in vivo. a TNC stable knockout A673 and SKNMC cell lines were established and confirmed by western blotting. b, c TNC-induced enhancement of ES cell (A673 and SKNMC cells) viability and migration, respectively (n = 4 wells per group). d, e Representative images and statistical analysis of TNC-induced promotion of angiogenesis (A673 and SKNMC cells, n = 3 wells per group). f Representative bioluminescence images at day 26. g In vivo bioluminescence images showing statistically significant differences 19 days after inoculation, with this difference peaking on day 26 (n = 8 mice per group). All in vitro experiments were repeated three times independently with at least three replicates per experiment (scale bars, 100 μm)