Fig. 5

Hsp60 knockdown inhibits TGF-β and IL-8 expression, whereas IL-8 enhances TGF-β. Hsp60-silenced and mock shRNA LNCaP cells were treated with Api, TG alone or in combination followed by IL-8 quantification using ELISA (a). Whole cell lysates were used to analyse Hsp60 knockdown using Western blotting. Actin serves as a loading control. b IL-8 knockdown was confirmed by ELISA in cell lysate. c Estimation of TGF-β in cell lysates using ELISA in mock and IL-8 knockdown LNCaP cells following treatment with Api or TG alone or their combination. d Estimation of TGF-β in cell lysates using ELISA in mock and Hsp60 knockdown LNCaP cells following treatment with Api or TG alone or their combination. Data are mean ± SD (n = 3). *p < 0.05 vs respective controls/groups