Fig. 6
C-MYC maintains SOX9 nuclear localisation in TAMR MCF-7 cells. a Representative confocal images of immunofluorescence for SOX9 location of shC-MYC-MCF-7 TAMR cells. Scale bar, 20 μm. b Western blot of SOX9 protein in cytoplasmic and nuclear fractions from shNC or shC-MYC TAMR MCF-7 cells. c Immunoprecipitation in shNC or shC-MYC-MCF-7 TAMR cells with anti-SOX9 followed by immunoblotting with an antibody against the acetylation proteins. d qRT-PCR analysis of HMGA2 and TCF4 mRNAs in TAMR MCF-7 cells transfected with shNC and shC-MYC. e MTT assay of growth rates of TAMR MCF-7 cells with shNC or shC-MYC. f MTT assay of growth rates of shC-MYC TAMR MCF-7 cells overexpressing HDAC5 or control vector. g Western blot of SOX9 protein in cytoplasmic and nuclear fractions from shC-MYC TAMR MCF-7 cells transfected with HDAC5 or control vector. h Representative confocal images for MYC-SOX9 location in shC-MYC TAMR MCF-7 cells with overexpression of SOX9 or KA-SOX9. i Analysis of the growth rates of KO-SOX9 TAMR MCF-7 cells with overexpressing WT-SOX9 or KA-SOX9 vector by MTT assay. Scale bar, 20 μm. Data are representative of means ± SEM of three independent experiments (unpaired t-test, *p < 0.05, **p < 0.01)
