Fig. 2 | British Journal of Cancer

Fig. 2

From: High-resolution imaging mass spectrometry combined with transcriptomic analysis identified a link between fatty acid composition of phosphatidylinositols and the immune checkpoint pathway at the primary tumour site of breast cancer

Fig. 2The alternative text for this image may have been generated using AI.

Replacement of PI(18:0/20:4) with PIs-MUFA as a common process of in situ breast cancer growth. a Left: the averaged FA composition of PIs detected in cancer cells and adjacent normal ductal cells. Right: the averaged FA composition of PIs detected in the surrounding stromal areas. * indicates a statistically significant difference in the comparison between normal ductal cells and non-invasive cancer cells or between non-invasive cancer cells and invasive cancer cells. Saturated, monounsaturated, di-unsaturated and polyunsaturated PIs are coloured in blue, yellow, red and green, respectively. Two-way ANOVA with Sidak’s post hoc multiple comparisons test. **p < 0.01; ***p < 0.001; ****p < 0.0001. b Principal component analysis of normal ductal cells and cancer cells based on the proportion of ten PIs. Left: each dot indicates an individual lesion. Blue: normal ductal cells; green: non-invasive cancer cells; red: invasive cancer cells. Right: the arrows indicate the contribution rate of PIs with different degrees of unsaturation. The contribution ratio of each component is displayed on the axis. c Representative histological mapping of PIs and the corresponding H&E staining. Upper panels: images of normal ductal cells and their associated stroma. Lower panels: images of non-invasive cancer cells and stroma. Normal ductal cells and cancer cells are encircled by a yellow dotted line in H&E staining. Relative intensity of ion signals is represented by an RGB scale. Scale bar (white): 50 μm. d Comparison of relative intensities between non-invasive cancer cells and normal ductal cells. White and black circles show individual lesions of normal ductal and non-invasive cancer cells, respectively. * indicates a statistically significant difference in the comparison between normal and non-invasive cancer cells. Two-way ANOVA with Sidak’s post hoc multiple comparisons test. **p < 0.01; ****p < 0.0001.

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