Fig. 3 | British Journal of Cancer

Fig. 3

From: High-resolution imaging mass spectrometry combined with transcriptomic analysis identified a link between fatty acid composition of phosphatidylinositols and the immune checkpoint pathway at the primary tumour site of breast cancer

Fig. 3The alternative text for this image may have been generated using AI.

Replacement of PIs-MUFA with PIs-PUFA occurs in invasive cancer cells and is recognised as PI(18:0/20:3) accumulation. a Inter-individual correlation of PIs with different FA compositions in invasive cancer cells. Red and blue circles indicate a positive and negative correlation, respectively. The circled values show the correlation coefficient of each combination, which is also represented by circle size. b Representative histological mapping and the corresponding H&E staining of invasive cancer cells and their surrounding stroma. Cancer cells are encircled by a yellow dotted line in H&E staining. “N” indicates a necrotic region. Relative intensity of ion signals is represented by an RGB scale. Scale bar (white): 50 μm. c Correlation of PIs-MUFA and PIs-PUFA in invasive cancer cells. Triangles and circles indicate individual lesions of invasive cancer cells. The presence of PI(18:0/20:3) accumulation in cancer cells is indicated as a purple triangle. d Comparison of the intensities of invasive cancer cells between the presence and absence of PI(18:0/20:3) accumulation. White and black circles indicate individual lesions for the presence or absence of PI(18:0/20:3) accumulation in cancer cells, respectively. * indicates a statistically significant difference in the comparison between normal and DCIS. Two-way ANOVA with Sidak’s post hoc multiple comparisons test. **p < 0.01; ***p < 0.001; ****p < 0.0001.

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