Fig. 5: Combinational TCP and GSK-J1 treatment induces hundreds of differentially expressed genes enriched in cancer-associated biological events and pathways.

a The heatmaps showing the differentially expressed genes by genome-wide transcriptional sequencing after TCP and GSK-J1 treatment in Cal27 cells. n = 2 biological replicates for each condition. b, c Gene ontology (b) and KEGG pathway (c) analyses of those differentially expressed genes revealed significant enrichment in cancer-related biological categories and pathways. d–g Gene set enrichment analysis (GSEA) of those differentially expressed genes revealed significant enrichment in apoptosis, cell cycle, cyclin D1 and p53 pathway.